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elmolino89

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elmolino89
·hace 2 meses·discuss
Lack of appropriate funding leads to cutting corners to the point that some results may not be worth the price of the paper to describe them. I had a passing experience with epigenetics. Even experiments with basically free of ethics issues cell lines could be screwed up by using single end, too short sequencing reads. Combined with too low coverage, less than perfect controls it gives the input data I which the state of the art peak callers will just throw the towel. So the "trick" is to use some way more forgiving peak caller and get a rather crappy results. Using the outdated human genome assembly (hg19), and old genome mapping programs just puts an extra cherry on the cake...
elmolino89
·hace 2 meses·discuss
I belive you are mixing assembling the genome by combining sequences of individual, overlapping inserts of cosmids, fosmids, PACs and BACs (bacterial vectors with human DNA inserts of 40-150kbp) to whole genome shotgun. The inserts of the above bacterial vectors were sequenced using shotgun, but the gaps in the sequence were closed with custom primers.
elmolino89
·hace 3 meses·discuss
Thank you for your kind words. Sorry for the Captain's Obvious a bit rude comment. It just happened that me and my partner were battling traffic using rickety road through the hills above the Barcelona to get on time for a chemotherapy session. Just to be told that due to the blackout it must be postponed for a few days. Luckily nothing serious in the long run, but nevertheless rather annoying. Because of the traffic jams and lack of mobile coverage for many hours in various locations in Spain I am quite sure there were some extra fatalities.

Thinking about it, there is nothing wrong writing about unexpected pleasures of that day. Just that we keep in mind the fact that blackouts have this non-hipstery, rather serious aspects.

PS I am doing ok-eish, splendid even considering the initial diagnosis (pancreatic cancer 1b stage diagnosed in December 2024). Maybe still way too touchy about some topic apparently triggering me.
elmolino89
·hace 3 meses·discuss
If you would have a family member having a scheduled chemotherapy treatment for that day the thrill would be gone. Not every day is great for the chill out.
elmolino89
·hace 3 meses·discuss
Spain does have few (probably 4 according to ESP Wikipedia) pumped-storage hydroelectricity plants. Supposedly these are being used nowadays to store excess of energy produced by fotovoltaic plants. No idea how fast these can switch from storing energy to producing it and if these were used to help during the blackout.
elmolino89
·hace 3 meses·discuss
Also missing in vast majority of the dams is a lower reservoir. Pumping up the water from a river/canal below the dam would result in a dry river bed just below the dam rather quickly

*edit* spell