Enzyme Electric Fields(science.org)
science.org
Enzyme Electric Fields
https://www.science.org/content/blog-post/enzyme-electric-fields
10 comments
Time resolved protein crystallography is a technique that achieves what you’re looking for.
https://en.wikipedia.org/wiki/Time_resolved_crystallography
https://youtu.be/wqYMA2rh8ow
https://youtu.be/OFnVsdFXifg
By the way, around 50% of the volume in protein crystals is usually occupied by water, with some cases reaching up to 80%.
https://en.wikipedia.org/wiki/Time_resolved_crystallography
https://youtu.be/wqYMA2rh8ow
https://youtu.be/OFnVsdFXifg
By the way, around 50% of the volume in protein crystals is usually occupied by water, with some cases reaching up to 80%.
You want molecular dynamics. They are simulations, not real images. But otoh (visible) light rays are "about as big" as a very large protein, so you can't image them. And because of computational expense they're slow motion by necessity, which is good because very, very complex enzyme interactions means ~2-3000 interactions per second.
Example: https://www.youtube.com/watch?v=vkYEYjintqU
Example: https://www.youtube.com/watch?v=vkYEYjintqU
I no longer work in the field, but I've always been incredibly impressed by the innovative methods that Steven Boxer uses in enzyme spectroscopy.
What about quantum chemical effects? Are they negligible? Why focus only on electrostatics?
What is the SOTA for computational models that predict enzymatic activity?
In pharmacology we have many enzyme inhibitors, that reduce their activity. Conversely, are there ways to either increase the rate of productivity of an enzyme, or to increase the number of enzyme of a type?
plz help
plz help
Many of them have probably tried hard for a long time at increasing efficacy. Other than addressing possible shortages of substrates and co-factors that seems like more trouble than it's worth most of the time?
More of the enzyme... Try homeopathy but don't dilute so much? (Production of inducible enzymes is upregulated based on environmental cues, often some variant of failing to work fast enough at catalyzing whatever they work on, ethanol being one popular example). The fancier way would be trying to find a suitable set of transcription factors to stimulate expression and get those into the right cells and compartments I guess.
More of the enzyme... Try homeopathy but don't dilute so much? (Production of inducible enzymes is upregulated based on environmental cues, often some variant of failing to work fast enough at catalyzing whatever they work on, ethanol being one popular example). The fancier way would be trying to find a suitable set of transcription factors to stimulate expression and get those into the right cells and compartments I guess.
I'm pretty sure there must be molecules or signaling pathways that upregulate specific enzymes but yeah as you say, epigenetically via specifically made peptides or genetically via mRNA injections, one could increase the quantity of a specific ensyme, the easyness being a product of how well delineated and specific are the genes/allele responsible for its encoding.
The medicinal applications are endless, e.g. increasing FAAH ensymes for next-gen sleep enhancers, or increasing specific ratios of phosphodiesterases for next-gen mood stabilisers/antypsychotics/anticonvulsants/antiepilleptics.
It seems like a very underfunded generic target.
Is their electrical conductance impacted by 5Ghz waves ?
I wonder if that would be possible?
You'd need some enzyme that can be crystallized and still work in a vacuum, and then a scanning electron microscope. While most enzymes need water, perhaps there are some that don't? The crystal keeps everything stationary. You'd scan a crystal of enzyme repeatedly while the reaction was going on.
Then you have the hard part... Figuring out a video of the enzyme reacting that is consistent with all the data sampled. Obviously what you're seeing is a large numbers of samples in time and space of the same thing happening repeatedly with unknown time offsets, and what you want is a video of the event happening just once. But that conversion ought to be possible... You might need to scan in a few different directions to help remove ambiguities...