pjin

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  After an appropriate transport period, the brain is
  perfused, sectioned, suitably stained, and each section
  digitally imaged. These 2-D images are co-registered into a
  3-D computer stack that is subsequently registered to a
  common reference atlas. The resulting 3-D brain image is
  largely unlabeled (i.e., contains no signal of interest),
  except for the connections between the injected region and
  its target regions. Thus the labeled connections are
  clearly identifiable. A given region is injected in
  multiple animals to account for individual variability.